Study of Anastasis in Yeast

A cell is said to be dead when the plasma membrane breaks down or the cell is fragmented in an irreversible way [1]. Cells can reach this state by two mutually exclusive events: accidental cell death (ACD) or programmed cell death (PCD). ACD is the result of exposure to extreme conditions (e.g., high temperature, ultrasound); PCD is the physiological response to mild conditions that involves the genetic response of cells. Hence, PCD may be reversed to prevent cells to die. In mammals this phenomenon is known as anastasis (from the Greek “rising to life”); this was discovered back in 2012 when cells exposed to a chemical that induces PCD was removed and these cells recovered [2]. Understanding the molecular mechanism of anastasis may assist in preserving injured cells that are difficult to replace (e.g., neurons, cardiomyocites).
The yeast Saccharomyces cerevisiae has been successfully used to study many molecular mechanisms relevant not only for fungi, but also for humans and other mammals because to its high degree of functional conservation, including cellular processes such as cell cycle [4], autophagy [5], epigentic control [6], among many others. In addition, several collections ready to use in high-throuput screenings to identify indispensable genes or molecular interactions associated to any cellular process of interest makes the yeast an ideal system to study molecular mechanisms. Thus, it would be convenient to develop an experimental system in yeast to study anastasis to accelerate the molecular understanding of it.

Principal Investigators
Klipp, Edda Prof. Dr. rer. nat. Dr. h.c. (Details) (Theoretical Biophysics)

Duration of Project
Start date: 06/2019
End date: 12/2019

Research Areas
Life Sciences

Last updated on 2020-06-11 at 20:03