Detection and functional characterization of queuosine and m5C modification in RNA

tRNAs are subject to a variety of chemical modifications that modulate their function in translation. In this project, we have investigated two such modifications, queuosine (Q) and cytosine-5 methylation (m5C). Our earlier work in Schizosaccharomyces pombe showed that Dnmt2-dependent methylation of C38 in tRNA-Asp is strongly stimulated by Q, which replaces guanosine at the wobble position (Q34) in the anticodon of selected tRNAs. Interestingly, Q in eukaryotes originally comes from bacterial sources in nutrition and from the gut microflora, thus providing a connection between nutritional supply and translation in the host. In this proposal, we will develop novel methods to detect Q and m5C in RNA. This project will yield novel tools for the detection of these modifications and will further improve our understanding of the epitranscriptome in eukaryotes.

Principal investigators
Ehrenhofer-Murray, Ann Prof. Dr. (Details) (Molecular Cell Biology)

Participating organisational units of HU Berlin

DFG: Sachbeihilfe

Duration of project
Start date: 11/2018
End date: 04/2022

Research Areas
Cell Biology

Research Areas

Last updated on 2022-07-09 at 19:06