Detection and functional characterization of queuosine and m5C modification in RNA


tRNAs are subject to a variety of chemical modifications that modulate their function in translation. In this project, we have investigated two such modifications, queuosine (Q) and cytosine-5 methylation (m5C). Our earlier work in Schizosaccharomyces pombe showed that Dnmt2-dependent methylation of C38 in tRNA-Asp is strongly stimulated by Q, which replaces guanosine at the wobble position (Q34) in the anticodon of selected tRNAs. Interestingly, Q in eukaryotes originally comes from bacterial sources in nutrition and from the gut microflora, thus providing a connection between nutritional supply and translation in the host. In this proposal, we will develop novel methods to detect Q and m5C in RNA. This project will yield novel tools for the detection of these modifications and will further improve our understanding of the epitranscriptome in eukaryotes.
tRNAs are subject to a variety of chemical modifications that modulate their function in translation. In this project, we have investigated two such modifications, queuosine (Q) and cytosine-5 methylation (m5C). Our earlier work in Schizosaccharomyces pombe showed that Dnmt2-dependent methylation of C38 in tRNA-Asp is strongly stimulated by Q, which replaces guanosine at the wobble position (Q34) in the anticodon of selected tRNAs. Interestingly, Q in eukaryotes originally comes from bacterial sources in nutrition and from the gut microflora, thus providing a connection between nutritional supply and translation in the host. In this proposal, we will develop novel methods to detect Q and m5C in RNA. This project will yield novel tools for the detection of these modifications and will further improve our understanding of the epitranscriptome in eukaryotes.


Principal investigators
Ehrenhofer-Murray, Ann Prof. Dr. (Details) (Molecular Cell Biology)

Participating organisational units of HU Berlin

Duration of project
Start date: 11/2018
End date: 04/2022

Research Areas
Cell Biology

Research Areas
Molekularbiologie

Last updated on 2022-07-09 at 19:06