NW: Analysis of pentatricopeptide repeat proteins in vivo: targets RNAs and functions II


We want to define targets and functions of selected PPR proteins. The focus is on a subset of PPRs, for which null-alleles in Arabidopsis have been shown to lead to an embryo-defective phenotype. These PPRs are likely to have a very basic function for chloroplast- and thus plant-development, at least in Arabidopsis. The work rests on two general approaches:



1. Definition of PPR RNA Targets


Only for very few RNA binding proteins have target RNAs been determined directly and if so, evidence was based on in vitro studies. We are using a novel approach that couples classical immunoprecipitation techniques with microarray analysis (RIP-Chip).We want to define targets and functions of selected PPR proteins. The focus is on a subset of PPRs, for which null-alleles in Arabidopsis have been shown to lead to an embryo-defective phenotype. These PPRs are likely to have a very basic function for chloroplast- and thus plant-development, at least in Arabidopsis. The work rests on two general approaches:



2. Functional Analysis via Reverse Genetics


It is a well-established fact that chloroplast development in grass species like maize is NOT essential for plant development, quite in contrast to dicot species like Arabidopsis. Therefore, PPR mutants for genes that are essential for embryo development in Arabidopsis will be sought in a maize mutant bank made available by Alice Barkan (http://chloroplast.uoregon.edu/). Recovered mutants will be analyzed for defects in plastid RNA metabolism, guided by RIP-Chip results.


Principal investigators
Schmitz-Linneweber, Christian Prof. Dr. (Details) (Junior Research Groups (Biology))

Financer
DFG: Nachwuchsgruppe

Duration of project
Start date: 11/2007
End date: 10/2009

Publications

Legen, J, Wanner, G, Herrmann, RG, Small, I, and Schmitz-Linneweber, C. 2007. Plastid tRNA Genes trnC-GCA and trnN-GUU are essential for plant cell development. Plant J. 51:751-762.



Schmitz-Linneweber C, Williams-Carrier R, Williams-Voelker PM, Kroeger, TS, Vichas, A, and Barkan A. 2006. A pentatricopeptide repeat protein facilitates the trans-splicing of the maize chloroplast rps12 pre-mRNA. Plant Cell. 18:2650-2663.



Tillich M, Poltnigg P, Kushnir S, and Schmitz-Linneweber C. 2006. Conservation of Plastid RNA editing factors independently of their target sites. EMBO Rep. 7(3):308-13.


Last updated on 2022-07-09 at 21:09