Using the Sunlight for Biocyatalysis – Development of Photo-Bioelectrode-Structures for Synthesis Isolation, Stabilization, Modification and Assembly of Photosystems and Its Components in Hybrid-Assemblies for Photo-Biocatalysis
The aim of the Tamdem-project is to develop light-activated electrode devices for biocatalytical purposes. Therefore photosystems or components should be combined with electrodes for an efficient photo-electron transfer. By coupling various enzyme systems with light-activated electrodes, the produced photocurrent enables the synthesis of valuable substances. To achieve this, functional hybrid-structures from anorganic and biological components should be applied on electrodes.
The two main objects of our project are i) coupling isolated photosystems or light-harvesting complexes with nano-based electrodes and ii) combination of those systems with biocatalytical reactions. Prerequisite for this is the efficient coupling of light-harvesting by the used protein complexes and generating photo-current at the electrode. Additionally, we are using generated charge carrier at the protein-electrode for further biochemical reactions. Based on this combining biocatalytical reactions with photo-activated electrodes results in light- and voltage guided synthesis of biochemical valuables.
Zouni, Athina PD Dr. (Details) (Honorary Chairs / Professors by Special Appointment / Part-Time Lecturers)
Financer
BMBF
Duration of project
Start date: 08/2013
End date: 10/2017
Research Areas
Basic Research in Biology and Medicine, Biophysics, Life Sciences, Molecular Chemistry, Natural Sciences, Physical and Theoretical Chemistry, Physical Chemistry of Molecules, Liquids and Interfaces, Biophysical Chemistry, Plant Biochemistry and Biophysics
Research Areas
Erneuerbare Energien, Membran
Publications
Müh F, DiFiore D, and Zouni A, Influence of poly(ethylene glycol) on the micelle formation of alkyl maltosides used in membrane protein crystallization, Physical Chemistry Chemical Physics 03/2015; 17(17). DOI: 10.1039/C5CP00431D
http://pubs.rsc.org/en/content/articlepdf/2015/cp/c5cp00431d
Stieger KR, Ciornii D, Kölsch A, Hejazi M, Lokstein H, Feifel SC, Zouni A, Lisdat F.Engineering of supramolecular photoactive protein architectures: the defined co-assembly of photosystem I and cytochrome c using a nanoscaled DNA-matrix. Nanoscale. 2016 May 19;8(20):10695-705. doi: 10.1039/c6nr00097e.
http://pubs.rsc.org/en/Content/ArticleLanding/2016/NR/C6NR00097E#!divAbstract
Stieger KR, Feifel SC, Lokstein H, Hejazi M, Zouni A, Lisdat F. Biohybrid architectures for efficient light-to-current conversion based on photosystem I within scalable 3D mesoporous electrodes. J. Mater. Chem. A, 2016, 4, 17009-17017 DOI: 10.1039/C6TA07141D
http://pubs.rsc.org/en/Content/ArticleLanding/2016/TA/C6TA07141D#!divAbstract
Golub M, Hejazi M, Kölsch A, Lokstein H, Wieland D.C.F, Zouni A, and Pieper J. Solution structure of monomeric and trimeric photosystem I of Thermosynechococcus elongatus investigated by small-angle X-ray scattering. Photosynthesis Research September 2017, Volume 133, Issue 1–3, pp 163–173. First Online: 03 March 2017
https://link.springer.com/article/10.1007/s11120-017-0342-6
Dmitri Ciornii, Feifel SC, Hejazi M, Kölsch A, Lokstein H, Zouni A, and Lisdat F. Construction of photobiocathodes using multi-walled carbon nanotubes and photosystem I. PSS. Phys. Status Solidi A 214, No. 9, 1700017 (2017) / DOI 10.1002/pssa.201700017. First published: 10 April 2017
http://onlinelibrary.wiley.com/doi/10.1002/pssa.201700017/full
