Effects of Root and Fungal Feeding Animal Consumers on Rhizosphere Interactions and Oak Performance

The proposed project investigates the gene expression response of mycorrhizal and non-mycorrhizal oak microcuttings to major rhizosphere animals, root and fungal feeding invertebrates, represented by nematodes and Collembola, respectively. The gene expression response of the plants will be investigated using micro-arrays and qRT-PCR technology in parallel to carbon allocation patterns using 13CO2 pulse labelling. Two growth phases of oak microcuttings i.e., root and shoot flush, and their response to root and fungal feeders in the rhizosphere will be studied. In a further experiment the time dependent flux of carbon into rhizosphere compartments including roots, soil microorganisms and fungal feeders will be investigated using bulk 13C and compound specific 13C lipid analysis. Both separate and combined effects of nematodes and Collembola will be studied. In addition, interactions between root feeding nematodes and beneficial rhizosphere bacteria (actinomycetes) will be investigated to obtain a comprehensive picture on rhizosphere microbial communities. The project aims at getting detailed insight into the genetic and C allocation response of oak microcuttings to animal rhizosphere consumers, and the implications of these interactions for rhizosphere microorganisms.

Principal Investigators
Rueß, Liliane Prof. Dr. (Details) (Ecology)

Duration of Project
Start date: 05/2010
End date: 09/2014

Last updated on 2020-04-11 at 13:03