Characterization of nutrient-dependent RNA methylation dynamics in S. pombe


In this project, the biological function of tRNA cytosine methylation by the tRNA methyltransferase Dnmt2 in the fission yeast Schizosaccharomyces pombe will be investigated. The project will encompass whole-transcriptome methylation analysis and ribosome profiling in order to determine how the chemical modification of tRNA and other RNA mioeties regulates translation. This will allow novel, transcriptome-wide insights into the function of an important RNA modification and will generate bioinformatic tools to expand this analysis towards other systems.


Principal Investigators
Ehrenhofer-Murray, Ann Prof. Dr. (Details) (Molecular Cell Biology)

Duration of Project
Start date: 06/2015
End date: 05/2018

Publications
Müller M, Legrand C, Tuorto F, Kelly VP, Atlasi Y, Lyko F and Ehrenhofer-Murray AE. Queuine links translational control in eukaryotes to a micronutrient from bacteria. (2019) Nucleic Acids Research, 47(7):3711-3727.

Müller M, Samel-Pommerencke A, Legrand C, Tuorto F, Lyko F, Ehrenhofer-Murray AE. (2019) Division of labour: tRNA methylation by the NSun2 tRNA methyltransferases Trm4a and Trm4b in fission yeast. RNA Biol. 2019 Jan 15. doi: 10.1080/15476286.2019.1568819.

Müller M, Hartmann M, Schuster I, Bender S, Thüring KL, Helm M, Katze JR, Nellen W, Lyko F, Ehrenhofer-Murray AE. (2015) Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine. Nucleic Acids Res. 2015 Dec 15;43(22):10952-62.

Tuorto F, Legrand C, Federico G, Liebers R, Cirzi C, Hofmann TG, Müller M, Ehrenhofer-Murray AE, Gröne HJ and Lyko F. (2018) Queuosine-modified tRNAs confer nutritional control of protein translation. EMBO J. 2018 Sep 14;37(18).

Becker M, Müller S, Nellen W, Jurkowski TP, Jeltsch A, Ehrenhofer-Murray AE. Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling. Nucleic Acids Res. 2012;40(22):11648-58.

Shanmugam R, Aklujkar M, Schäfer M, Reinhardt R, Nickel O, Reuter G, Lovley DR, Ehrenhofer-Murray AE, Nellen W, Ankri S, Helm M, Jurkowski TP, Jeltsch A. (2014) The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu. Nucleic Acids Res. 2014 Jun;42(10):6487-96.

Ehrenhofer-Murray AE. Cross-Talk between Dnmt2-Dependent tRNA Methylation and Queuosine Modification. Biomolecules. 2017 Feb 10;7(1). pii: E14.

Jeltsch A, Ehrenhofer-Murray A, Jurkowski TP, Lyko F, Reuter G, Ankri S, Nellen W, Schaefer M, Helm M. Mechanism and biological role of Dnmt2 in Nucleic Acid Methylation. RNA Biol. 2016 May 27:1-16.

Last updated on 2020-12-11 at 12:24