CRC 740/3: Acetylation of Lysine 16 on Histone H4 as a Dynamic Epigenetic Mark During Transcription and Replication (SP A07)


Chromatin packaging of DNA ensures proper compaction and organization of the genetic material in the eukaryotic nucleus, but at the same time restricts its accessibility to DNA regulatory processes like transcription, replication and DNA repair. One principle way of altering chromatin function is by posttranslational modification of the histones that make up the nucleosomes. One such modification, the acetylation of lysine 16 of histone H4 (H4 K16Ac), will be the focus of this study, because it conveys special properties to chromatin in that it inhibits heterochromatin formation by altering the compaction of the chromatin fibre and by counteracting the binding of the repressive silent information regulator (SIR) complex in yeast.


Principal Investigators
Ehrenhofer-Murray, Ann Prof. Dr. (Details) (Molecular Cell Biology)

Duration of Project
Start date: 01/2015
End date: 12/2018

Publications
Reiter, C, Heise, F, Chung, HR, Ehrenhofer-Murray AE (2015) A link between Sas2-mediated H4 K16 acetylation, chromatin assembly in S-phase by CAF-I and Asf1, and nu-cleosome assembly by Spt6 during transcription. FEMS Yeast Res. 2015 Nov;15(7). pii: fov073.

Anedchenko EA, Samel-Pommerencke A, Tran Nguyen TM, Shahnejat-Bushehri S, Pöpsel J, Lauster D, Herrmann A, Rappsilber J, Cuomo A, Bonaldi T, Ehrenhofer-Murray AE. The kinetochore module Okp1CENP-Q/Ame1CENP-U is a reader for N-terminal modifications on the centromeric histone Cse4CENP-A. EMBO J. 2019 Jan 3;38(1). pii: e98991. doi: 10.15252/embj.201898991. Epub 2018 Nov 2.

Last updated on 2020-18-11 at 14:13