SPP 1784: Charakterisierung der Nährstoff-abhängigen Dynamik von RNA-Methylierung in S. pombe

In this project, the biological function of tRNA cytosine methylation by the tRNA methyltransferase Dnmt2 in the fission yeast Schizosaccharomyces pombe will be investigated. The project will encompass whole-transcriptome methylation analsis and ribosome profiling in order to determine how the chemical modification of tRNA and other RNA mioeties regulates translation. This will allow novel, transcriptome-wide insights into the function of an important RNA modification and will generate bioinformatics tools to expand this analysis towards other systems.

Projektleitung
Ehrenhofer-Murray, Ann Prof. Dr. (Details) (Molekulare Zellbiologie)

Mittelgeber
DFG: Sachbeihilfe

Laufzeit
Projektstart: 06/2015
Projektende: 05/2018

Publikationen
Müller M, Legrand C, Tuorto F, Kelly VP, Atlasi Y, Lyko F and Ehrenhofer-Murray AE. Queuine links translational control in eukaryotes to a micronutrient from bacteria. (2019) Nucleic Acids Research, 47(7):3711-3727.

Müller M, Samel-Pommerencke A, Legrand C, Tuorto F, Lyko F, Ehrenhofer-Murray AE. (2019) Division of labour: tRNA methylation by the NSun2 tRNA methyltransferases Trm4a and Trm4b in fission yeast. RNA Biol. 2019 Jan 15. doi: 10.1080/15476286.2019.1568819.

Müller M, Hartmann M, Schuster I, Bender S, Thüring KL, Helm M, Katze JR, Nellen W, Lyko F, Ehrenhofer-Murray AE. (2015) Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine. Nucleic Acids Res. 2015 Dec 15;43(22):10952-62.

Tuorto F, Legrand C, Federico G, Liebers R, Cirzi C, Hofmann TG, Müller M, Ehrenhofer-Murray AE, Gröne HJ and Lyko F. (2018) Queuosine-modified tRNAs confer nutritional control of protein translation. EMBO J. 2018 Sep 14;37(18).

Becker M, Müller S, Nellen W, Jurkowski TP, Jeltsch A, Ehrenhofer-Murray AE. Pmt1, a Dnmt2 homolog in Schizosaccharomyces pombe, mediates tRNA methylation in response to nutrient signaling. Nucleic Acids Res. 2012;40(22):11648-58.

Shanmugam R, Aklujkar M, Schäfer M, Reinhardt R, Nickel O, Reuter G, Lovley DR, Ehrenhofer-Murray AE, Nellen W, Ankri S, Helm M, Jurkowski TP, Jeltsch A. (2014) The Dnmt2 RNA methyltransferase homolog of Geobacter sulfurreducens specifically methylates tRNA-Glu. Nucleic Acids Res. 2014 Jun;42(10):6487-96.

Ehrenhofer-Murray AE. Cross-Talk between Dnmt2-Dependent tRNA Methylation and Queuosine Modification. Biomolecules. 2017 Feb 10;7(1). pii: E14.

Jeltsch A, Ehrenhofer-Murray A, Jurkowski TP, Lyko F, Reuter G, Ankri S, Nellen W, Schaefer M, Helm M. Mechanism and biological role of Dnmt2 in Nucleic Acid Methylation. RNA Biol. 2016 May 27:1-16.

Zuletzt aktualisiert 2020-19-06 um 15:54