SFB 852-I: The effect of dietary supplementation of Enterococcus faecium NCIMB 10415 and zinc on signaling gene regulation in the immune response of mesenteric lymph nodes in piglets

Dietary supplementation of probiotics as well as zinc is supposed to improve health in weaning piglets and to protect against infections. Our aim is to identify mechanisms contributing to expected improved defence as result of dietary supplementation. We will examine whole genome gene expression and study signalling pathways in the immune system in mesenteric lymph nodes in piglets untreated or treated with either Enterococcus faecium NCIMB 10415 or zinc. Our data will be analyzed together with data from other projects to find systems biological correlations between all parameters.

Brockmann, Gudrun A. Prof. Dr. rer. nat. habil. (Details) (Züchtungsbiologie und molekulare Tierzüchtung)

DFG: Sonderforschungsbereich - Beteiligungen

Projektstart: 01/2010
Projektende: 12/2014

1. Kreuzer S, Janczyk P, Assmus J, Schmidt MF, Brockmann GA, Nockler K: No Beneficial Effects Evident for Enterococcus faecium NCIMB 10415 in Weaned Pigs Infected with Salmonella enterica Serovar Typhimurium DT104. Applied and environmental microbiology 2012, 78(14):4816-4825.

2. Kreuzer S, Machnowska P, Assmus J, Sieber M, Pieper R, Schmidt MF, Brockmann GA, Scharek-Tedin L, Johne R: Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs. Veterinary research 2012, 43(1):58.

3. Kreuzer S, Reissmann M, Brockmann GA: Gene test to elucidate the ETEC F4ab/F4ac receptor status in pigs. Veterinary microbiology 2012.

4. Janczyk P, Kreuzer S, Assmus J, Nockler K, Brockmann GA: No protective effects of high-dosage dietary zinc oxide on weaned pigs infected with Salmonella enterica serovar typhimurium DT104. Applied and environmental microbiology 2013, 79(9):2914-2921.

5. Kreuzer S, Reissmann M, Brockmann GA: New fast and cost-effective gene test to get the ETEC F18 receptor status in pigs. Veterinary microbiology 2013, 163(3-4):392-394.

6. Kreuzer S, Rieger J, Strucken EM, Thaben N, Hunigen H, Nockler K, Janczyk P, Plendl J, Brockmann GA: Characterization of CD4+ subpopulations and CD25+ cells in ileal lymphatic tissue of weaned piglets infected with Salmonella Typhimurium with or without Enterococus faecium feeding. Veterinary immunology and immunopathology 2014, 158(3-4):143-155.

7. Siepert B, Reinhardt N, Kreuzer S, Bondzio A, Twardziok S, Brockmann GA, Nöckler K, Szabo I, Janczyk P, Pieper R et al: Enterococcus faecium NCIMB 10415 supplementation affects intestinal immune-associated gene expression in post-weaning piglets and may delay immune responses to Salmonella infections. Veterinary Immunology Immunopathology 2014, 157:65-77.

8. Twardziok SO, Pieper R, Aschenbach JR, Bednorz C, Brockmann GA, Fromm M, Klingspor S, Kreuzer S, Lodemann U, Martens H et al: Cross- talk Between Host, Microbiome and Probiotics: A Systems Biology Approach for Analyzing the Effects of Probiotic Enterococcus faecium NCIMB 10415 in Piglets. Molecular Informatics 2014, 33(3):171-182.

9. Karweina D, Kreuzer-Redmer S, Muller U, Franken T, Pieper R, Baron U, Olek S, Zentek J, Brockmann GA: The Zinc Concentration in the Diet and the Length of the Feeding Period Affect the Methylation Status of the ZIP4 Zinc Transporter Gene in Piglets. PLoS One 2015, 10(11):e0143098.

10. Kreuzer-Redmer S, Bekurtz JC, Arends D, Bortfeldt R, Kutz-Lohroff B, Sharbati S, Einspanier R, Brockmann GA: Feeding of Enterococcus faecium NCIMB 10415 Leads to Intestinal miRNA-423-5p-Induced Regulation of Immune-Relevant Genes. Applied and environmental microbiology 2016, 82(8):2263-2269.

11. Schulte JN, Brockmann GA, Kreuzer-Redmer S: Feeding a high dosage of zinc oxide affects suppressor of cytokine gene expression in Salmonella Typhimurium infected piglets. Veterinary immunology and immunopathology 2016, 178:10-13.

12. Kreuzer-Redmer S, Arends D, Schulte JN, Karweina D, Korkuc P, Woltje N, Hesse D, Pieper R, Gerdts V, Zentek J et al: High dosage of zinc modulates T-cells in a time-dependent manner within porcine gut-associated lymphatic tissue. Br J Nutr 2018, 120(12):1349-1358.

13. Karweina D, Martin L, Pieper R, Zentek J, Brockmann GA: Epigenetic effects of dietary zinc on the porcine ZIP4 gene expression. Perspectives in Science 2015, 3(1–4):18-20.

Zuletzt aktualisiert 2020-26-10 um 14:05